headshot of Thomas Smithgall

Thomas Smithgall

Professor

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Emert-Sedlak, L.A., Tice, C.M., Shi, H., Alvarado, J.J., Shu, S.T., Reitz, A.B., & Smithgall, T.E. (2024). PROTAC-mediated degradation of HIV-1 Nef efficiently restores cell-surface CD4 and MHC-I expression and blocks HIV-1 replication. Cell Chem Biol, 31(4), 658-668.e14.Elsevier. doi: 10.1016/j.chembiol.2024.02.004.

Heinrich, F., Thomas, C.E., Alvarado, J.J., Eells, R., Thomas, A., Doucet, M., Whitlatch, K.N., Aryal, M., Lösche, M., & Smithgall, T.E. (2023). Neutron Reflectometry and Molecular Simulations Demonstrate HIV-1 Nef Homodimer Formation on Model Lipid Bilayers. J Mol Biol, 435(8), 168009.Elsevier. doi: 10.1016/j.jmb.2023.168009.

Du, S., Alvarado, J.J., Wales, T.E., Moroco, J.A., Engen, J.R., & Smithgall, T.E. (2022). ATP-site inhibitors induce unique conformations of the acute myeloid leukemia-associated Src-family kinase, Fgr. Structure, 30(11), 1508-1517.e3.Elsevier. doi: 10.1016/j.str.2022.08.008.

Li, W.F., Aryal, M., Shu, S.T., & Smithgall, T.E. (2020). HIV-1 Nef dimers short-circuit immune receptor signaling by activating Tec-family kinases at the host cell membrane. J Biol Chem, 295(15), 5163-5174.Elsevier. doi: 10.1074/jbc.RA120.012536.

Staudt, R.P., & Smithgall, T.E. (2020). Nef homodimers down-regulate SERINC5 by AP-2-mediated endocytosis to promote HIV-1 infectivity. J Biol Chem, 295(46), 15540-15552.Elsevier. doi: 10.1074/jbc.RA120.014668.

Staudt, R.P., Alvarado, J.J., Emert-Sedlak, L.A., Shi, H., Shu, S.T., Wales, T.E., Engen, J.R., & Smithgall, T.E. (2020). Structure, function, and inhibitor targeting of HIV-1 Nef-effector kinase complexes. J Biol Chem, 295(44), 15158-15171.Elsevier. doi: 10.1074/jbc.REV120.012317.

Moroco, J.A., Alvarado, J.J., Staudt, R.P., Shi, H., Wales, T.E., Smithgall, T.E., & Engen, J.R. (2018). Remodeling of HIV-1 Nef Structure by Src-Family Kinase Binding. J Mol Biol, 430(3), 310-321.Elsevier. doi: 10.1016/j.jmb.2017.12.008.

Shen, K., Moroco, J.A., Patel, R.K., Shi, H., Engen, J.R., Dorman, H.R., & Smithgall, T.E. (2018). The Src family kinase Fgr is a transforming oncoprotein that functions independently of SH3-SH2 domain regulation. Sci Signal, 11(553), eaat5916.American Association for the Advancement of Science (AAAS). doi: 10.1126/scisignal.aat5916.

Shu, S.T., Emert-Sedlak, L.A., & Smithgall, T.E. (2017). Cell-based Fluorescence Complementation Reveals a Role for HIV-1 Nef Protein Dimerization in AP-2 Adaptor Recruitment and CD4 Co-receptor Down-regulation. J Biol Chem, 292(7), 2670-2678.Elsevier. doi: 10.1074/jbc.M116.770016.

Emert-Sedlak, L.A., Loughran, H.M., Shi, H., Kulp, J.L., Shu, S.T., Zhao, J., Day, B.W., Wrobel, J.E., Reitz, A.B., & Smithgall, T.E. (2016). Synthesis and evaluation of orally active small molecule HIV-1 Nef antagonists. Bioorg Med Chem Lett, 26(5), 1480-1484.Elsevier. doi: 10.1016/j.bmcl.2016.01.043.

Grover, P., Shi, H., Baumgartner, M., Camacho, C.J., & Smithgall, T.E. (2015). Fluorescence Polarization Screening Assays for Small Molecule Allosteric Modulators of ABL Kinase Function. In Buday, L. (Ed.). PLoS One, 10(7), e0133590.Public Library of Science (PLoS). doi: 10.1371/journal.pone.0133590.

Alvarado, J.J., Tarafdar, S., Yeh, J.I., & Smithgall, T.E. (2014). Interaction with the Src homology (SH3-SH2) region of the Src-family kinase Hck structures the HIV-1 Nef dimer for kinase activation and effector recruitment. J Biol Chem, 289(41), 28539-28553.Elsevier. doi: 10.1074/jbc.M114.600031.

Iyer, P.C., Zhao, J., Emert-Sedlak, L.A., Moore, K.K., Smithgall, T.E., & Day, B.W. (2014). Synthesis and structure-activity analysis of diphenylpyrazolodiazene inhibitors of the HIV-1 Nef virulence factor. Bioorg Med Chem Lett, 24(7), 1702-1706.Elsevier. doi: 10.1016/j.bmcl.2014.02.045.

Moroco, J.A., Craigo, J.K., Iacob, R.E., Wales, T.E., Engen, J.R., & Smithgall, T.E. (2014). Differential sensitivity of Src-family kinases to activation by SH3 domain displacement. In Sticht, H. (Ed.). PLoS One, 9(8), e105629.Public Library of Science (PLoS). doi: 10.1371/journal.pone.0105629.

Tarafdar, S., Poe, J.A., & Smithgall, T.E. (2014). The accessory factor Nef links HIV-1 to Tec/Btk kinases in an Src homology 3 domain-dependent manner. J Biol Chem, 289(22), 15718-15728.Elsevier. doi: 10.1074/jbc.M114.572099.

Zhang, X., Simerly, C., Hartnett, C., Schatten, G., & Smithgall, T.E. (2014). Src-family tyrosine kinase activities are essential for differentiation of human embryonic stem cells. Stem Cell Res, 13(3 Pt A), 379-389.Elsevier. doi: 10.1016/j.scr.2014.09.007.

Perrone, R., Nadai, M., Poe, J.A., Frasson, I., Palumbo, M., Palù, G., Smithgall, T.E., & Richter, S.N. (2013). Formation of a unique cluster of G-quadruplex structures in the HIV-1 Nef coding region: implications for antiviral activity. In Qiu, J. (Ed.). PLoS One, 8(8), e73121.Public Library of Science (PLoS). doi: 10.1371/journal.pone.0073121.

Trible, R.P., Narute, P., Emert-Sedlak, L.A., Alvarado, J.J., Atkins, K., Thomas, L., Kodama, T., Yanamala, N., Korotchenko, V., Day, B.W., Thomas, G., & Smithgall, T.E. (2013). Discovery of a diaminoquinoxaline benzenesulfonamide antagonist of HIV-1 Nef function using a yeast-based phenotypic screen. Retrovirology, 10(1), 135.Springer Nature. doi: 10.1186/1742-4690-10-135.

Narute, P.S., & Smithgall, T.E. (2012). Nef alleles from all major HIV-1 clades activate Src-family kinases and enhance HIV-1 replication in an inhibitor-sensitive manner. In Vartanian, J.P. (Ed.). PLoS One, 7(2), e32561.Public Library of Science (PLoS). doi: 10.1371/journal.pone.0032561.

Pene-Dumitrescu, T., Shu, S.T., Wales, T.E., Alvarado, J.J., Shi, H., Narute, P., Moroco, J.A., Yeh, J.I., Engen, J.R., & Smithgall, T.E. (2012). HIV-1 Nef interaction influences the ATP-binding site of the Src-family kinase, Hck. BMC Chem Biol, 12(1), 1.Springer Nature. doi: 10.1186/1472-6769-12-1.

O'Reilly, L.P., Watkins, S.C., & Smithgall, T.E. (2011). An unexpected role for the clock protein timeless in developmental apoptosis. In Pesce, M. (Ed.). PLoS One, 6(2), e17157.Public Library of Science (PLoS). doi: 10.1371/journal.pone.0017157.