Mohammad Ataai

  • Ph.D., Chemical Engineering, Cornell University, 1986
  • M.S., Chemical Engineering, Tufts University, 1981

  • Meade, J., Bartlow, P., Trivedi, R.N., Akhtar, P., Ataai, M.M., Khan, S.A., & Domach, M.M. (2015). Effect of plasmid replication deregulation via inc mutations on E. coli proteome & simple flux model analysis. Microb Cell Fact, 14(1), 31.Springer Nature. doi: 10.1186/s12934-015-0212-x.
  • Haley, R., Fruchtl, M., Brune, E.M., Ataai, M., Henry, R., & Beitle, R. (2014). A redesigned Escherichia coli triosephosphate isomerase restores growth properties in a bacterial strain useful for Immobilized Metal Affinity Chromatography (IMAC). J Biotechnol, 188, 48-52.Elsevier. doi: 10.1016/j.jbiotec.2014.07.432.
  • Trivedi, R.N., Akhtar, P., Meade, J., Bartlow, P., Ataai, M.M., Khan, S.A., & Domach, M.M. (2014). High-Level Production of Plasmid DNA by Escherichia coli DH5α ΩsacB by Introducing inc Mutations. In Parales, R.E. (Ed.). Appl Environ Microbiol, 80(23), 7154-7160.American Society for Microbiology. doi: 10.1128/AEM.02445-14.
  • Bartlow, P., Tiwari, N., Beitle, R.R., & Ataai, M.M. (2012). Evaluation of Escherichia coli proteins that burden nonaffinity-based chromatography as a potential strategy for improved purification performance. Biotechnol Prog, 28(1), 137-145.Wiley. doi: 10.1002/btpr.703.
  • Meade, J., Khan, S., Ataai, M., & Domach, M. (2012). Use of flux pre-analysis to enable ¹³C tracer studies in pyruvate kinase-deficient Escherichia coli. Biotechnol J, 7(3), 449-460.Wiley. doi: 10.1002/biot.201100338.
  • Bartlow, P., Uechi, G.T., Cardamone, J.J., Sultana, T., Fruchtl, M., Beitle, R.R., & Ataai, M.M. (2011). Identification of native Escherichia coli BL21 (DE3) proteins that bind to immobilized metal affinity chromatography under high imidazole conditions and use of 2D-DIGE to evaluate contamination pools with respect to recombinant protein expression level. Protein Expr Purif, 78(2), 216-224.Elsevier. doi: 10.1016/j.pep.2011.04.021.
  • Pan, Z., Cunningham, D.S., Zhu, T., Ye, K., Koepsel, R.R., Domach, M.M., & Ataai, M.M. (2010). Enhanced recombinant protein production in pyruvate kinase mutant of Bacillus subtilis. Appl Microbiol Biotechnol, 85(6), 1769-1778.Springer Nature. doi: 10.1007/s00253-009-2244-9.
  • Tiwari, N., Woods, L., Haley, R., Kight, A., Goforth, R., Clark, K., Ataai, M., Henry, R., & Beitle, R. (2010). Identification and characterization of native proteins of Escherichia coli BL-21 that display affinity towards Immobilized Metal Affinity Chromatography and Hydrophobic Interaction Chromatography Matrices. Protein Expr Purif, 70(2), 191-195.Elsevier. doi: 10.1016/j.pep.2009.10.018.
  • Cunningham, D.S., Koepsel, R.R., Ataai, M.M., & Domach, M.M. (2009). Factors affecting plasmid production in Escherichia coli from a resource allocation standpoint. Microb Cell Fact, 8(1), 27.Springer Nature. doi: 10.1186/1475-2859-8-27.
  • Cunningham, D.S., Liu, Z., Domagalski, N., Koepsel, R.R., Ataai, M.M., & Domach, M.M. (2009). Pyruvate kinase-deficient Escherichia coli exhibits increased plasmid copy number and cyclic AMP levels. J Bacteriol, 191(9), 3041-3049.American Society for Microbiology. doi: 10.1128/JB.01422-08.
  • Dilmore, R., Griffith, C., Liu, Z., Soong, Y., Hedges, S.W., Koepsel, R., & Ataai, M. (2009). Carbonic anhydrase-facilitated CO2 absorption with polyacrylamide buffering bead capture. INTERNATIONAL JOURNAL OF GREENHOUSE GAS CONTROL, 3(4), 401-410.Elsevier. doi: 10.1016/j.ijggc.2009.01.004.
  • Grant, K.G., Krisky, D.M., Ataai, M.M., & Glorioso, J.C. (2009). Engineering cell lines for production of replication defective HSV-1 gene therapy vectors. Biotechnol Bioeng, 102(4), 1087-1097.Wiley. doi: 10.1002/bit.22123.
  • Liu, Z., Bartlow, P., Dilmore, R.M., Soong, Y., Pan, Z., Koepsel, R., & Ataai, M. (2009). Production, Purification, and Characterization of a Fusion Protein of Carbonic Anhydrase from Neisseria gonorrhoeae and Cellulose Binding Domain from Clostridium thermocellum. BIOTECHNOLOGY PROGRESS, 25(1), 68-74.Wiley. doi: 10.1002/btpr.80.
  • Liu, Z., Bartlow, P., Varakala, R., Beitle, R., Koepsel, R., & Ataai, M.M. (2009). Use of proteomics for design of a tailored host cell for highly efficient protein purification. J Chromatogr A, 1216(12), 2433-2438.Elsevier. doi: 10.1016/j.chroma.2009.01.020.
  • Ghosh, S., Grossmann, I.E., Ataai, M.M., & Domach, M.M. (2006). A three-level problem-centric strategy for selecting NMR precursor labeling and analytes. Metab Eng, 8(5), 491-507.Elsevier. doi: 10.1016/j.ymben.2006.05.001.
  • Jiang, C., Ataai, M., Ozuer, A., Krisky, D., Wechuck, J., Pornsuwan, S., Pourarian, F., & Glorioso, J.C. (2006). Inactivation of herpes simplex type 1 gene vector on immobilized metal affinity chromatography: oxidative damage by hydroxyl free radicals and its prevention. Biotechnol Bioeng, 95(1), 48-57.Wiley. doi: 10.1002/bit.20943.
  • Jiang, C., Gloioso, J.C., & Ataai, M.M. (2006). Presence of Imidazole in Loading Buffer Prevents Formation of Free Radical in IMAC and Dramatically Improves the Recovery of HSV-1 Gene Therapy Vectors. J. Chromatography A, J Chromatogr A, 1121(1), 40-45.
  • Jiang, C., Glorioso, J.C., & Ataai, M. (2006). Presence of imidazole in loading buffer prevents formation of free radical in immobilized metal affinity chromatography and dramatically improves the recovery of herpes simplex virus type 1 gene therapy vectors. J Chromatogr A, 1121(1), 40-45.Elsevier. doi: 10.1016/j.chroma.2006.04.071.
  • Pan, Z., Zhu, T., Domagalski, N., Khan, S., Koepsel, R.R., Domach, M.M., & Ataai, M.M. (2006). Regulating expression of pyruvate kinase in Bacillus subtilis for control of growth rate and formation of acidic byproducts. BIOTECHNOLOGY PROGRESS, 22(5), 1451-1455.Wiley. doi: 10.1021/bp060049u.
  • Pan, Z., Zhu, T., Domagalski, N., Khan, S., Koepsel, R.R., Domach, M.M., & Ataai, M.M. (2006). Regulating expression of pyruvate kinase in Bacillus subtilis for control of growth rate and formation of acidic byproducts. Biotechnol Prog, 22(5), 1451-1455. doi: 10.1021/bp060049u.
  • Ghosh, S., Zhu, T., Grossmann, I.E., Ataai, M.M., & Domach, M.M. (2005). Closing the loop between feasible flux scenario identification for construct evaluation and resolution of realized fluxes via NMR. COMPUTERS & CHEMICAL ENGINEERING, 29(3), 459-466.Elsevier. doi: 10.1016/j.compchemeng.2004.08.027.
  • Zhu, T., Pan, Z., Domagalski, N., Koepsel, R., Ataai, M.M., & Domach, M.M. (2005). Engineering of Bacillus subtilis for enhanced total synthesis of folic acid. Appl Environ Microbiol, 71(11), 7122-7129.American Society for Microbiology. doi: 10.1128/AEM.71.11.7122-7129.2005.
  • Jiang, C., Wechuck, J.B., Goins, W.F., Krisky, D.M., Wolfe, D., Ataai, M.M., & Glorioso, J.C. (2004). Immobilized cobalt affinity chromatography provides a novel, efficient method for herpes simplex virus type 1 gene vector purification. J Virol, 78(17), 8994-9006.American Society for Microbiology. doi: 10.1128/JVI.78.17.8994-9006.2004.
  • Ye, K., Jin, S., Ataai, M.M., & Schultz, S. (2004). Tagging retrovirus with a metal binding peptide: a novel approach for preparation of clinical-grade retroviral vectors by immobilized metal affinity chromatography purification. J. Virol., 78, 9820-9827.
  • Ye, K., Jin, S., Ataai, M.M., Schultz, J.S., & Ibeh, J. (2004). Tagging retrovirus vectors with a metal binding peptide and one-step purification by immobilized metal affinity chromatography. J Virol, 78(18), 9820-9827.American Society for Microbiology. doi: 10.1128/JVI.78.18.9820-9827.2004.
  • Zhu, T., Ataai, M.M., Domach, M.M., & Grossmann, I.E. (2003). A metabolic network analysis & NMR experimental design program with user interface-driven model construction. Metabolic Engineering, 5, 74-85.
  • Zhu, T., Grossmann, I.E., Domach, M.M., & Ataai, M.M. (2003). Theoretical improvement of folic acid production in E. coli. Fermentation Biotechnology, ACS symposium series, 862, 207-219.
  • Zhu, T., Phalakornkule, C., Ghosh, S., Grossmann, I.E., Koepsel, R.R., Ataai, M.M., & Domach, M.M. (2003). A metabolic network analysis & NMR experiment design tool with user interface-driven model construction for depth-first search analysis. Metab Eng, 5(2), 74-85.Elsevier. doi: 10.1016/s1096-7176(03)00023-5.
  • Lee, J., Goel, A., Ataai, M.M., & Domach, M.M. (2002). Flux regulation patterns and energy audit of E-coli B/r and K-12. JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 12(2), 258-267.
  • Ozuer, A., Wechuck, J.B., Russell, B., Wolfe, D., Goins, W.F., Glorioso, J.C., & Ataai, M.M. (2002). Evaluation of Infection Parameters in the Production of HSV-1 Viral Vectors For Gene Therapy. Biotech. Prog., 18, 476-482.
  • Ozuer, A., Wechuck, J.B., Russell, B., Wolfe, D., Goins, W.F., Glorioso, J.C., & Ataai, M.M. (2002). Evaluation of infection parameters in the production of replication-defective HSV-1 viral vectors. Biotechnol Prog, 18(3), 476-482.Wiley. doi: 10.1021/bp010176k.
  • Ozuer, A., Wetchuck, J.C., Goins, W.F., Wolfe, D., Glorioso, J.C., & Ataai, M.M. (2002). Effect of Genetic Background, Culture Conditions, and Reactor Configuration on Productivity of HSV-1 Vectors. Biotechnol. Bioeng., 77, 685-692.
  • Wechuck, J.B., Ozuer, A., Goins, W.F., Wolfe, D., Oligino, T., Glorioso, J.C., & Ataai, M.M. (2002). Effect of temperature, medium composition, and cell passage on production of herpes-based viral vectors. Biotechnol Bioeng, 79(1), 112-119.Wiley. doi: 10.1002/bit.10310.
  • Phalakornkule, C., Lee, S., Zhu, T., Koepsel, R., Ataai, M.M., Grossmann, I.E., & Domach, M.M. (2001). A MILP-based flux alternative generation and NMR experimental design strategy for metabolic engineering. Metab Eng, 3(2), 124-137.Elsevier. doi: 10.1006/mben.2000.0165.
  • Ward, M.S., Ataai, M., Koepsel, R.R., & Shepherd, R.E. (2001). Comparison of energy-minimized structures of [Pd(II)(N-methyliminodiacetate)] complexes of X(1)-His-X(3)-His-His peptides as an analysis of steric and specific interactions with synthetic binding tags for IMAC separations. Biotechnol Prog, 17(4), 712-719.Wiley. doi: 10.1021/bp010050n.
  • Zhu, T., Phalakornkule, C., Koepsel, R.R., Domach, M.M., & Ataai, M.M. (2001). Cell growth and by-product formation in a pyruvate kinase mutant of E. coli. Biotechnol Prog, 17(4), 624-628.Wiley. doi: 10.1021/bp0100575.
  • Chen, Y., Pasquinelli, R., Ataai, M., Koepsel, R.R., Kortes, R.A., & Shepherd, R.E. (2000). Coordination of two high-affinity hexamer peptides to copper(II) and palladium(II) models of the peptide-metal chelation site on IMAC resins. Inorg Chem, 39(6), 1180-1186.American Chemical Society (ACS). doi: 10.1021/ic990520z.
  • Fry, B., Zhu, T., Domach, M.M., Koepsel, R.R., Phalakornkule, C., & Ataai, M.M. (2000). Characterization of growth and acid formation in a Bacillus subtilis pyruvate kinase mutant. Appl Environ Microbiol, 66(9), 4045-4049.American Society for Microbiology. doi: 10.1128/AEM.66.9.4045-4049.2000.
  • Fry, B., Zhu, T., Phalakornkule, C., Koepsel, R., Domach, M.M., & Ataai, M.M. (2000). Characterization of Growth and Acid Formation in a Bacillus subtilis Pyruvate Kinase mutant. Appl Environ. Microbiol, 66, 1045-1049.
  • Lee, S., Phalakornkule, C., Ataai, M.M., Domach, M.M., & Grossmann, I.E. (2000). Recursive MILP Model for Finding All the Alternate Optima in LP Models for Metabolic Networks. Computers in Chemical Engineering, 39, 1180-1186.
  • Pasquinelli, R.S., Shepherd, R.E., Koepsel, R.R., Zhao, A., & Ataai, M.M. (2000). Design of affinity tags for one-step protein purification from immobilized zinc columns. Biotechnol Prog, 16(1), 86-91.Wiley. doi: 10.1021/bp990139h.
  • Phalakornkule, C., Fry, B., Zhu, T., Kopesel, R., Ataai, M.M., & Domach, M.M. (2000). 13C NMR evidence for pyruvate kinase flux attenuation underlying suppressed acid formation in Bacillus subtilis. Biotechnol Prog, 16(2), 169-175.Wiley. doi: 10.1021/bp000007k.
  • Wechuck, J.B., Goins, W.F., Glorioso, J.C., & Ataai, M.M. (2000). Effect of protease inhibitors on yield of HSV-1-based viral vectors. Biotechnol Prog, 16(3), 493-496.Wiley. doi: 10.1021/bp000016t.
  • Wu, N., & Ataai, M.M. (2000). Production of viral vectors for gene therapy applications. Curr Opin Biotechnol, 11(2), 205-208.Elsevier. doi: 10.1016/s0958-1669(00)00080-x.
  • Chen, Y., Pasquinelli, R., Ataai, M., Koepsel, R.R., Stringfield, T.W., & Shepherd, R.E. (1999). Coordination of the six-mer peptide Gly-His-Pro-His-His-Gly to CuII and PdIIN-methyliminodiacetate complexes as IMAC chelation site models. JOURNAL OF INORGANIC BIOCHEMISTRY, 76(3-4), 211-220.Elsevier. doi: 10.1016/S0162-0134(99)00129-4.
  • Goel, A., Lee, J., Domach, M.M., & Ataai, M.M. (1999). Metabolic fluxes, pools, and enzyme measurements suggest a tighter coupling of energetics and biosynthetic reactions associated with reduced pyruvate kinase flux. Biotechnol Bioeng, 64(2), 129-134.Wiley. doi: 10.1002/(sici)1097-0290(19990720)64:2<129::aid-bit1>3.0.co;2-i.
  • Goel, A., Lee, J., Domach, M.M., & Ataai, M.M. (1999). Metabolic fluxes, pools, and enzyme measurements suggest a tighter coupling of energetics and biosynthetic reactions associated with reduced pyruvate kinase flux. Biotechnology and Bioengineering, 64(2), 129-134. doi: 10.1002/(SICI)1097-0290(19990720)64:2<129::AID-BIT1>3.0.CO;2-I.
  • Pasquinelli, R.S., Koepsel, R.R., Wu, N., & Ataai, M.M. (1999). Vector engineering anomalies: impact on fusion protein purification performance. Protein Expr Purif, 17(3), 449-455.Elsevier. doi: 10.1006/prep.1999.1152.
  • Owen, M.J., & Karsenty, G. (1998). New developments in bone formation. 7, 363-366.Wolters Kluwer. doi: 10.1097/00041552-199807000-00003.
  • Patwardhan, A., Pascquinelli, R., Koepsel, R., & Ataai, M.M. (1998). Phage-displayed libraries for the selection of optimal affinity tails for Ni-NTA purification of recombinant proteins. Biotechnology Techniques, 12, 421-424.
  • Patwardhan, A.V., Goud, G.N., Pasquinelli, R.S., Koepsel, R.R., & Ataai, M.M. (1998). Phage-displayed libraries for the selection of optimal affinity peptides for protein purification using Ni-nitrilotriacetic Acid chromatography. BIOTECHNOLOGY TECHNIQUES, 12(6), 421-424.Springer Nature. doi: 10.1023/A:1008842902587.
  • Bhatia, R., Jesionowski, G., Ferrance, J., & Ataai, M.M. (1997). Insect cell physiology. Cytotechnology, 24(1), 1-9.Springer Nature. doi: 10.1023/B:CYTO.0000010410.24541.dd.
  • Bhatia, R., Jesionowski, G., Ferrance, J., & Ataai, M.M. (1997). Insect cell physiology. Cytotechnology, 24(1), 1-9.Springer Science and Business Media LLC. doi: 10.1023/b:cyto.0000010410.24541.dd.
  • Bhatia, R., Jesionowski, G., Ferrance, J., & Ataai, M.M. (1997). Erratum: Insect cell physiology (Cytotechnology (1996) 20 (33-41)). Cytotechnology, 24(1), 87.
  • Goud, G.N., Patwardhan, A.V., Beckman, E.J., Ataai, M.M., & Koepsel, R.R. (1997). Selection of specific peptide ligands for immobilized metals using a phage displayed library: Application to protein separation using IMAC. International Journal of Bio-Chromatography, 3(2), 123-136.
  • Goud, N., Patwardhan, A., Koepsel, R., & Ataai, M.M. (1997). Selection of specific peptide ligands for Immobilized Cu (II) using a phage displayed peptide library on bacteriophage. Intl. J. Biochromatography, 3, 123-136.
  • Jesionowski, G., & Ataai, M.M. (1997). Drastic improvements in insect cell/reccombinat baculovirus protein productivity cultured in a physiologically designed medium. Biotechnology Progress, 13, 355-360.
  • Jesionowski, G.A., & Ataai, M.M. (1997). An efficient medium for high protein production in the insect cell baculovirus expression system. BIOTECHNOLOGY PROGRESS, 13(4), 355-360.Wiley. doi: 10.1021/bp970054l.
  • Lee, J., Goel, A., Ataai, M.M., & Domach, M.M. (1997). optimality of co-feeding of carbon sources for maximizing cellular and energetic yields using a constrained network analysis. Appl Environ. Microbiol., 63, 710-718.
  • Lee, J., Goel, A., Ataai, M.M., & Domach, M.M. (1997). Supply-Side Analysis of Growth of Bacillus subtilis on Glucose-Citrate Medium: Feasible Network Alternatives and Yield Optimality. Appl Environ Microbiol, 63(2), 710-718.American Society for Microbiology. doi: 10.1128/aem.63.2.710-718.1997.
  • Lee, J., Goel, A., Ataai, M.M., & Domach, M.M. (1997). Supply-Side Analysis of Growth of Bacillus subtilis on Glucose-Citrate Medium: Feasible Network Alternatives and Yield Optimality. Applied and Environmental Microbiology, 63(5), 2109.American Society for Microbiology. doi: 10.1128/aem.63.5.2109-2109.1997.
  • Patwardhan, A.V., & Ataai, M.M. (1997). Site accessibility and the pH dependence of the saturation capacity of a highly cross-linked matrix. Immobilized metal affinity chromatography of bovine serum albumin on chelating Superose. J Chromatogr A, 767(1-2), 11-23.Elsevier. doi: 10.1016/s0021-9673(97)00013-7.
  • Patwardhan, A.V., & Ataai, M.M. (1997). Mathematical modeling of protein purification of E. coli cell extract using immobilized metal affinity chromatography: Effect of loading pH on the column capacity and purity of the target protein. AIChE J, 43, 3232-3240.
  • Patwardhan, A.V., & Ataai, M.M. (1997). IMA chromatography of a cell extract: Effect of loading pH on protein purification. AICHE JOURNAL, 43(12), 3232-3240.Wiley. doi: 10.1002/aic.690431211.
  • Patwardhan, A.V., Goud, G.N., Koepsel, R.R., & Ataai, M.M. (1997). Selection of optimum affinity tags from a phage-displayed peptide library. Application to immobilized copper(II) affinity chromatography. J Chromatogr A, 787(1-2), 91-100.Elsevier. doi: 10.1016/s0021-9673(97)00580-3.
  • Wagner, W.R., Muzzio, D.J., Rilo, H.R., Deglau, T., Ataai, M.M., Michalopoulos, G.K., & Block, G.D. (1997). Effect of growth factors and defined medium on primary hepatocyte culture on polyester carriers with varying surface treatment. TISSUE ENGINEERING, 3(3), 289-301.SAGE Publications. doi: 10.1089/ten.1997.3.289.
  • Wagner, W.R., Muzzio, D.J., Rilo, H.R., Deglaus, T., & Ataai, M.M. (1997). Effect of Growth Factors and Defined Medium on Primary Hepatocyte Culture on Polyester Carriers With Varying Surface Treatments. Tissue Engineering, 3, 298-301.
  • Bhatia, R., Jesionowski, G., Ferrance, J., & Ataai, M.M. (1996). Insect cell physiology. 20, (pp. 33-41).Springer Nature. doi: 10.1007/BF00350387.
  • Goel, A., Domach, M.M., Hanley, W., Lee, J.W., & Ataai, M.M. (1996). Coordination of glycolysis and TCA cycle reaction networks. Citrate-glucose cometabolism eliminates acids and reveals potential metabolic engineering strategies. Ann N Y Acad Sci, 782(1), 1-16.Wiley. doi: 10.1111/j.1749-6632.1996.tb40542.x.
  • Hauck, J.T., Olson, G.J., Scierka, S.J., Perry, M.B., & Ataai, M.M. (1996). Effects of simulated flue gas on growth of microalgae. ACS Division of Fuel Chemistry, Preprints, 41(4), 1391-1394.
  • Parwardhan, A.V., & Ataai, M.M. (1996). One step purification of human interleukin 1b using IMAC. J. Biochromatography, 2, 119-124.
  • Patwardhan, A.V., & Ataai, M.M. (1996). One step purification of human interleukin-1β from the cellular protein of Escherichia coli using the immobilized metal affinity chromatography. International Journal of Bio-Chromatography, 2(2), 119-124.
  • Patwardhan, A.V., Ataai, M.M., & Zenouzi, M. (1996). Mathematical modeling and optimization of the immobilized metal affinity chromatography. American Society of Mechanical Engineers, Bioengineering Division (Publication) BED, 33, 223-224.
  • Goel, A., Lee, J., Domach, M.M., & Ataai, M.M. (1995). Suppressed acid formation by cofeeding of glucose and citrate in Bacillus cultures: emergence of pyruvate kinase as a potential metabolic engineering site. Biotechnol Prog, 11(4), 380-385.Wiley. doi: 10.1021/bp00034a003.
  • Parwardhan, A.V., Zenouzi, M., & Ataai, M.M. (1995). Design of efficient loading and elution strategies for IMAC separations: purification of human interleukin 1B from E. coli cell extract. AIChE Proceedings On Recent Development and Future Opportunities In Separation Technology, 1, 418-424.
  • Patwardhan, A.V., Ataai, M.M., & Zenouzi, M. (1995). Mathematical model of the immobilized metal affinity chromatography for protein separations: Simulation of loading and elution conditions. American Society of Mechanical Engineers, Heat Transfer Division, (Publication) HTD, 322, 61-68.
  • Vierheller, C., Goel, A., Peterson, M., Domach, M.M., & Ataai, M.M. (1995). Sustained and constitutive high levels of protein production in continuous cultures of bacillus subtilis. Biotechnol Bioeng, 47(5), 520-524.Wiley. doi: 10.1002/bit.260470503.
  • Lee, J., Goel, A., Ataai, M.M., & Domach, M.M. (1994). Flux adaptation of a citrate synthase mutant of E. coli. Ann. NY. Acad. Sci., 745, 35-59.
  • Lee, J., Goel, A., Ataai, M.M., & Domach, M.M. (1994). Flux adaptations of citrate synthase-deficient Escherichia coli. Ann N Y Acad Sci, 745(1), 35-50.Wiley. doi: 10.1111/j.1749-6632.1994.tb44362.x.
  • Almeida, N.F., Beckman, E.J., & Ataai, M.M. (1993). Immobilization of glucose oxidase in thin polypyrrole films: influence of polymerization conditions and film thickness on the activity and stability of the immobilized enzyme. Biotechnol Bioeng, 42(9), 1037-1045.Wiley. doi: 10.1002/bit.260420904.
  • Beitle, R.R., & Ataai, M.M. (1993). One-step purification of a model periplasmic protein from inclusion bodies by its fusion to an effective metal-binding peptide. Biotechnol Prog, 9(1), 64-69.Wiley. doi: 10.1021/bp00019a009.
  • Beitle, R.R., & Ataai, M.M. (1993). An effective, modular interfacing network for the monitoring of culture parameters: Acquisition of data from several RS-232 devices. Process Control and Quality, 4(2), 99-107.
  • Beitle, R.R., Sagar, S.L., Ataai, M.M., & Domach, M.M. (1993). Metal-based affinity separation of a- and b-chymotrypsin. Bioseparation, 3, 291-296.
  • Ferrance, J.P., Goel, A., & Ataai, M.M. (1993). Utilization of glucose and amino acids in insect cell cultures: Quantifying the metabolic flows within the primary pathways and medium development. Biotechnol Bioeng, 42(6), 697-707.Wiley. doi: 10.1002/bit.260420604.
  • Goel, A., Ferrance, J., Jeong, J., & Ataai, M.M. (1993). Analysis of metabolic fluxes in batch and continuous cultures of Bacillus subtilis. Biotechnol Bioeng, 42(6), 686-696.Wiley. doi: 10.1002/bit.260420603.
  • Sagar, S.L., Beitle, R.R., Ataai, M.M., & Domach, M.M. (1992). Metal-based affinity separation of alpha- and gamma-chymotrypsin and thermal stability analysis of isolates. Bioseparation, 3(5), 291-296.
  • BEITLE, R.R., & ATAAI, M.M. (1991). THE PURIFICATION OF TEM-BETA-LACTAMASE FROM CELL EXTRACT USING IMMOBILIZED COPPER AFFINITY-CHROMATOGRAPHY. BIOTECHNOLOGY TECHNIQUES, 5(2), 107-110.Springer Nature. doi: 10.1007/BF00159980.
  • BEITLE, R.R., & ATAAI, M.M. (1991). A UNIQUE MONITORING AND CONTROL-SYSTEM FOR LABORATORY FERMENTERS. BIOTECHNOLOGY TECHNIQUES, 5(2), 77-82.Springer Nature. doi: 10.1007/BF00159974.
  • Jeong, J., & Ataai, M.M. (1990). A highly structured model for simulation of batch and continuous cultures of B. subtilis and examination of cellular differentiation. Ann N Y Acad Sci, 589(1), 82-90.Wiley. doi: 10.1111/j.1749-6632.1990.tb24236.x.
  • Jeong, J.W., Snay, J., & Ataai, M.M. (1990). A Model For Examining Growth and Sporulation Processes of B. subtilis. Biotechnol. Bioeng., 35, 160-184.
  • Jeong, J.W., Snay, J., & Ataai, M.M. (1990). A mathematical model for examining growth and sporulation processes of Bacillus subtilis. Biotechnol Bioeng, 35(2), 160-184.Wiley. doi: 10.1002/bit.260350208.
  • SNAY, J., JEONG, J.W., & ATAAI, M.M. (1989). EFFECTS OF GROWTH-CONDITIONS ON CARBON UTILIZATION AND ORGANIC BY-PRODUCT FORMATION IN B-SUBTILIS. BIOTECHNOLOGY PROGRESS, 5(2), 63-69.Wiley. doi: 10.1002/btpr.5420050207.
  • Longo, A., Snay, J., Zimmer, L., & Ataai, M.M. (1988). Calcium Dependence of Proteolytic Activity of Residual Protease of B. subtilis. Biotechnology Letters, 10, 649-654.
  • LONGO, A., SNAY, J.R., ZIMMER, L., & ATAAI, M.M. (1988). CALCIUM REQUIREMENTS OF RESIDUAL PROTEASE IN BACILLUS-SUBTILIS DB104. BIOTECHNOLOGY LETTERS, 10(9), 649-654.Springer Nature. doi: 10.1007/BF01024719.
  • Ataai, M.M., & Shuler, M.L. (1987). A Mathematical Model for Prediction of Plasmid Copy Number and Genetic Stability in E. coli,. Biotechnol. Bioeng., 30(389-397).
  • Ataai, M.M., & Shuler, M.L. (1987). A mathematical model for prediction of plasmid copy number and genetic stability in Escherichia coli. Biotechnol Bioeng, 30(3), 389-397.Wiley. doi: 10.1002/bit.260300310.
  • Kim, B.G., Good, T.A., Ataai, M.M., & Shuler, M.L. (1987). Growth behavior and prediction of copy number and retention of ColE1-type plasmids in E. coli under slow growth conditions. Ann N Y Acad Sci, 506(1), 384-395.Wiley. doi: 10.1111/j.1749-6632.1987.tb23835.x.
  • Ataai, M.M., & Shuler, M.L. (1986). Mathematical model for the control of ColE1 type plasmid replication. Plasmid, 16(3), 204-212.Elsevier. doi: 10.1016/0147-619x(86)90058-2.
  • Ataai, M.M., & Shuler, M.L. (1985). Simulation of the growth pattern of a single cell of Escherichia coli under anaerobic conditions. Biotechnol Bioeng, 27(7), 1027-1035.Wiley. doi: 10.1002/bit.260270714.
  • Ataai, M.M., & Shuler, M.L. (1985). Simulation of CFSTR through development of a mathematical model for anaerobic growth of Escherichia coli cell population. Biotechnol Bioeng, 27(7), 1051-1055.Wiley. doi: 10.1002/bit.260270717.
  • Lee, A.L., Ataai, M.M., & Shuler, M.L. (1984). Double-substrate-limited growth of escherichia coli. Biotechnol Bioeng, 26(11), 1398-1401.Wiley. doi: 10.1002/bit.260261120.
  • FROHLICH, B.T., WEBSTER, I.A., ATAAI, M.M., & SHULER, M.L. (1983). PHOTOBIOREACTORS - MODELS FOR INTERACTION OF LIGHT-INTENSITY, REACTOR DESIGN, AND ALGAL PHYSIOLOGY. BIOTECHNOLOGY AND BIOENGINEERING, 13(13), 331-350.

  • Liu, Z., Bartlow, P., Dilmore, R.M., Soong, Y., Pan, Z., Koepsel, R., & Ataai, M. (2009). Production, purification, and characterization of a fusion protein of carbonic anhydrase from Neisseria gonorrhoeae and cellulose binding domain from Clostridium thermocellum. AIChE mtg.United States. doi: 10.1002/btpr.80.
  • Grant, K.G., Krisky, D.M., Ataai, M.M., & Glorioso, J.C.I.I.I. (2006). Engineering Cell Lines for the Production of Highly Replication Defective HSV-1 Vectors. International Herpesvirus Workshop.Asheville, NC. doi: 10.1016/j.ymthe.2006.08.1216.
  • Domagalski, N.R., Pan, Z.W., Zhu, T., Ye, K.M., Koepsel, R., Ataai, M.M., & Domach, M.M. (2005). Combining enhanced traits of E-coli into B-subtilis. ACS Natl Mtg.San Diego, CA.
  • Grant, K.G., Ataai, M.M., Glorioso, J.C., & Krisky, D. (2005). Cell line development for production of clinical grade HSV vectors. ACS Natl Mtg.San Diego, CA.
  • Haley, R., Henry, R., Goforth, R., Ataai, M.M., & Beitle, R.R. (2005). Circumventing the effects of high binding immobilized metal affinity chromatography contaminants. AICHE 2005 annual meeting.Cincinnati, OH.
  • Jiang, C., Wechuck, J., Ozuer, A., Glorioso, J., & Ataai, M. (2005). Reducing agents and imidazole protect Hsv-1 vector from inactivation during immobilized cobalt affinity chromatography. AICHE 2005 annual meeting.Cincinnati, OH.
  • Jiang, C.P., Wechuck, J.B., Krisky, D., Glorioso, J.C., & Ataai, M. (2005). Immobilized cobalt affinity purification for HSV-1 gene therapy vector. ACS Natl Mtg.San Diego, CA.
  • Pan, Z.W., Zhu, T., Domagalski, N.R., Koepsel, R., Domach, M., & Ataai, M.M. (2005). Regulating expression of PYK in B subtilis to reduce formation of acidic by-products and improve production of folic acid and recombinant protein. ACS Natl Mtg.San Diego, CA.
  • Ye, K.M., Jin, S., Ataai, M., & Schultz, J. (2005). One-step purification of retrovirus vectors by IMAC. ACS Natl Mtg.San Diego, CA.
  • Ataai, M., Jiang, C.P., Krisky, D.M., Goins, W.F., & Glorioso, J.C. (2004). Developing vero cell line expressing cobalt affinity peptide tagged HSV-1 GC for delivering the tag on the virus surface facilitating the virus purification. ACS Natl Mtg.Anaheim, CA.
  • Zhu, T., Koepsel, R., Domach, M.M., & Ataai, M. (2003). Metabolic engineering of folic acid production. ACS National Meeting.Boston, MA.
  • Zhu, T., Koepsel, R., Domach, M.M., & Ataai, M.M. (2003). Metabolic engineering of folic acid production. ACS National Meeting.New Orleans.
  • Ozuer, A., Wechuck, J.B., Goins, W.F., Wolfe, D., Glorioso, J.C., & Ataai, M.M. (2002). Effect of genetic background and culture conditions on the production of herpesvirus-based gene therapy vectors. European Society of Gene Therapy.United States. doi: 10.1002/bit.10162.
  • Ozuer, A., Wechuck, J.B., Wolfe, D., Goins, F., Wendell, S., Glorioso, J.C., & Ataai, M.M. (2002). Production of replication-defective HSV-1 viral vectors and development of rapid, and precise assay systems to analyze defective and infective particles. ACS National Meeting.Boston, MA.
  • Ozuer, A., Wechuck, J.B., Wolfe, D., Goins, W.F., Wendell, S., Glorioso, J.C., & Ataai, M.M. (2002). Efficient production and analysis of clinical-grade HSV-1 based gene therapy vectors. ACS National Meeting.Boston, MA.
  • Lee, S., Phalakornkule, C., Domach, M.M., & Grossmann, I.E. (2000). Recursive MILP model for finding all the alternate optima in LP models for metabolic networks. ACS Natl Mtg.San Francisco, CA. doi: 10.1016/S0098-1354(00)00323-9.
  • Chen, Y., Pasquinelli, R.S., Ataai, M.M., Koepsel, R.R., & Sheperd, R.E. (1998). Study of the coordination of the high affinity six-mer peptide Ser-Pro-His-His-Gly-Gly to a [PdII(methyliminodiacetate)] model of the CuII(iminodiacetate) chromatographic binding site. ACS Natl. Mtg..Boston.
  • Wechuck, J.B., Oligino, T., Glorioso, J.C., & Ataai, M.M. (1998). Efficient strategies for high titer production of viral vectors: Hsv-1/vero cells as a case study. ACS Natl. Mtg..Boston.
  • Ataai, M.M., Holder, G.D., & Toplak, R.F. (1997). Research experience for undergraduates at the University of Pittsburgh department of chemical and petroleum engineering. In Proceedings - Frontiers in Education Conference, 3, (pp. 1140-1145).
  • Beitle, R.R., & Ataai, M.M. (1992). Immobilized metal affinity chromatography and related techniques. In AIChE Symposium Series, 88(290), (pp. 34-44).
  • Ataai, M.M. (1986). MATHEMATICAL MODEL FOR REPLICATION OF R1 PLASMIDS. In American Institute of Chemical Engineers, National Meeting.
  • Ataai, M.M., & Shuler, M.L. (1985). MATHEMATICAL MODEL FOR PREDICTION OF COPY NUMBER AND PROTEIN PRODUCTION IN E. COLI. In Annual Meeting - American Institute of Chemical Engineers.
  • Shuler, M.L., Ataai, M.M., & Good, T.A. (1984). COMPUTER MODELS OF INDIVIDUAL BACTERIAL CELLS: A POSSIBLE TOOL FOR TESTING BIOCHEMICAL HYPOTHESES. In Annual Meeting - American Institute of Chemical Engineers.
Research Interests
Industrial biotechnology Biochemistry and cell biology Microbiology Medical biotechnology Medical microbiology Chemical engineering
headshot of Mohammad Ataai
Contact Mohammad Ataai
Professor Emeritus
ataai@pitt.edu
Work: 412-624-9648
Fax: 412-624-9639
Chem/Petroleum Engineering