Matthew Wolf

Faculty Mentor: Stephen Badylak

Research:Mr. Wolf's research focuses on the characterization of extracellular matrix (ECM) scaffolds composed of cardiac muscle. ECM scaffolds represent the secreted product of resident cells of each tissue and organ (including the heart), and have been increasingly shown to play critical instructive and inductive roles in tissue regeneration. Although cardiac ECM has been evaluated in terms of the changes that occur following injury and in pathologic conditions, the role of cardiac ECM in the context of cardiac regeneration has been only minimally explored. The research conducted during this training program will comprehensively characterize decellularized cardiac muscle extracellular matrix. The characterization will include quantitative analysis of major constituents including collagens, growth factors, and glycosaminoglycan content. Structural analysis will include light microscopy and immunohistochemistry, scanning electron microscopy, and time of flight SIMS analysis. Biologically active molecules will include generation of degradation products that would occur during remodeling of the ECM and their potential chemotactic and mitogenic properties upon cardiomyocytes, and progenitor cells. This ECM analysis will be used to create an inductive scaffold for the repair and reconstruction of functional myocardial tissue.  

Publications:  

• Brown BN, Freund JM, Han L, Rubin JP, Reing JE, Jeffries EM, Wolf MT, Tottey S, Barnes CA, Ratner BD, Badylak SF.. "Comparison of Three Methods for the Derivation of a Biologic Scaffold Composed of Adipose Tissue Extracellular Matrix.".
• Reing JE, Brown BN, Daly KA, Freund JM, Gilbert TW, Hsiong SX, Huber A, Kullas KE, Tottey S, Wolf MT, Badylak SF.. "The effects of processing methods upon mechanical and biologic properties of porcine dermal extracellular matrix scaffolds.".  
• Bhashyam AR, Wolf MT, Marcinkowski AL, Saville A, Thomas K, Carcillo JA, Corcoran TE. "Aerosol delivery through nasal cannulas: an in vitro study.". J Aerosol Med Pulm Drug Deliv21(2): 181-8, 2008 
• Wolf MT, Daly KA, Reing JE, Badylak SF.. "Biologic scaffold composed of skeletal muscle extracellular matrix". Biomaterials33(10): 2916-25, 2012 
• Daly KA, Wolf M, Johnson SA, Badylak SF.. "A rabbit model of peripheral compartment syndrome with associated rhabdomyolysis and a regenerative medicine approach for treatment.". Tissue Eng Part C Methods17(6): 631-40, 2011 
• Brown BN, Londono R, Tottey S, Zhang L, Kukla KA, Wolf MT, Daly KA, Reing JE, Badylak SF.. "Macrophage phenotype as a predictor of constructive remodeling following the implantation of biologically derived surgical mesh materials.". Acta Biomaterialia2011(2011): , 2011 

Conferences:  

• Bioengineering Graduate Student Research Symposium, Pittsburgh, PA, United States of America, 2007.
• ATACCC Conference, St. Petersburg, FL, USA, 08/2009
• BMES 2009, Pittsburgh, PA, USA, 10/2009
• AFIRM all hands meeting, St. Petersburg, FL, USA, 01/2010
• Sixth Symposium on Biologic Scaffolds for Regenerative Medicine, Napa Valley, CA, USA, 04/2010
• BMES 2010, Austin, TX, USA, 10/2010
• American Society for Matrix Biology Meeting, Charleston, SC, USA, 10/2010
• Society for Biomaterials, Orlando, FL, USA, 05/2011
• TERMIS NA, Houston, TX, USA, 12/2011
• American Society for Matrix Biology, San Diego, CA. USA, 12/2012
• Uniformed Services University of the Health Sciences (USUHS) Research Days, Bethesda, MD. USA, 1/2013
• Society for Biomaterials, Boston, MA. USA, 1/2013
• 17th Annual Hilton Head Regenerative Medicine Workshop, Hilton Head, SC. USA, 1/2013
• WFPC AFIRM Enabling Technologies Workshop for Young Minds, Pittsburgh, PA. USA, 1/2013
• McGowan Institute for Regenerative Medicine Annual Retreat, Nemacolin, PA. USA, 1/2013

 

Matthew Wolf is a graduate student in Dr. Stephen F. Badylak's lab, and is currently investigating the production and characterization of biologic scaffolds composed of cardiac and skeletal muscle extracellular matrix (ECM).